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concerns. According to statistics, one in eight. Characterization of strains by microarray. purchased from Bio-diagnostic chemical company.. As expected, the concentrations of soluble forms of adhesion molecules in all groups of obese women were significantly higher than these achieved in the lean control, but the highest ones were observed in the type 2 diabetes mellitus group. In patients with impaired fasting glucose their concentrations reach values higher than in the NFG subgroup, but the increase was not statistically significant. The positive correlation between the concentrations of glucose and sICAM-1 was noted only in T2DM group. This is consistent with the observations of Ziccardi et al.  who emphasize the role of high glucose levels in an intensification of adhesion molecules expression. Additionally, a positive correlation was observed between HOMA-IR and the concentrations of E-selectin, in T2DM subgroup. This correlation is especially significant, since E-selectin synthesized de novo by the endothelium activating factors, is a molecule expressed only by endothelial cells. Similar results were obtained in the multiple regression analysis carried out in the whole group of obese women. Thus, the results obtained in this study may, in some extent, reflect the relationship between the severity of endothelial dysfunction, glycemic status and insulin resistance degree. Similar observations were performed by Song et al. . It was pointed in other research that an increase in sICAM-1 and E-selectin levels is most pronounced in patients with T2DM . Also Mizia-Stec et al.  noted higher levels of E-selectin in patients with stable coronary artery disease and type 2 diabetes. Furthermore, in a nested case-control substudy within the prospective Nurses' Health Study revealed that high concentrations of E-selectin and sICAM-1 are the independent predictors for development of T2DM in non-diabetic women . More importantly, the levels of E-selectin in the prospective studies proved to be a stronger predictor of T2DM than sICAM-1 and sVCAM-1 . Additionally, an increase in serum levels of E-selectin allows to predict cardiovascular events in patients with type 2 diabetes . In turn, the reverse correlation between the concentrations of sICAM-1, E-selectin and HDL-cholesterol demonstrated in T2DM group should be mainly associated with protective effect of HDL-cholesterol on the endothelial function confirmed in numerous in vitro and in vivo studies [49,50]. Another observed association in the T2DM subgroup was a positive correlation between the triglycerides and sVCAM-1 levels. It is consistent with recent research by Wang et al. , which showed that circulating triglyceride-rich lipoproteins and endoplasmic reticulum stress modulate the expression of adhesion molecules, in particular VCAM-1, which plays an important role in the development of endothelial inflammation. Gustavson et al.  pointed out that high levels of glucose and cholesterol are an important factor stimulating the expression of VCAM-1 in retinal vessels in ApoE-/-mice. In turn, Ghoneim et al.  demonstrated that an increase in sICAM and sVCAM concentrations is closely related to the development of neuropathy, nephropathy or retinopathy in patients with DM. Metabolic disturbances in diabetes play an important role in the development of endothelial dysfunction and contribute to the creation of complications such as micro-and macroangiopathy . The relationship between VCAM-1 expression in the endothelium of carotid artery and atherosclerosis plaque progression and the occurrence of coronary artery disease (CAD) was demonstrated in animal model studies .. Cellular mechanisms involved in the behavior changes of SD rats induced by Homer 1a RNAi. the pelvis as possible, careful.
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The results are reported as the means and standard deviations of three independent analysis experiments, for western blot analysis, the experiments were repeated three times with corresponding blots from 4 different tissue slices. Statistical analysis was performed using one-way ANOVA. *P<0.05, **P<0.01 or ***P<0.001: the mean values were significantly different from those of the N group, #P<0.05, ##P<0.01 or ###P<0.001: the mean values were significantly different from those of the HC group..