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To improve the transduction efficiency of PMSCs, Lipofectamine 2000 was used to mediate adenoviral transduction. As shown in Fig. 2A, a very significant increase in the expression of EGFP in PMSCs was observed when the cells were transfected with Lipofectamine 2000, and a high-level viral titer resulted in enhanced expression of EGFP. Furthermore, flow cytometry was used to determine the transduction efficiency, in which 29.7% of PMSCs cells expressed EGFP at an MOI of 1500. This value was increased to 66.3% at an MOI of 3000 using Lipofectamine 2000 (Fig. 2B). These data were consistent with the results obtained from microscopic quantification. On the basis of these results, we selected an MOI of 3000 mixed with Lipofectamine 2000 at a ratio of 2.5×108 vp/µg for subsequent experiments..
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